Goat anti-DAX1 / NR0B1 Antibody

SKU: orb19492

Description

Goat polyclonal antibody to NR0B1

Images & Validation

• Tested Applications: ELISA, FC, IF, WB
• Dilution Range: ELISA: 1:32000, WB: 0.5-1.5 μg/ml
• Reactivity: Human
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 50-55kDa band observed in human ovary and human testis lysates (calculated MW of 51.7kDa according to NP_000466.2). Recommended concentration: 0.5-1.5 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: DAX1 / NR0B1
• Protein Sequence: CGEDHPQQGSTLY
• Molecular Weight: 51.7
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti NR0B1 antibody, anti DAX1 antibody, anti nuclear receptor subfamily 0, group B, member 1 antibody, anti AHC antibody, anti AHCH antibody, anti AHX antibody, anti DAX-1 antibody, anti DSS antibody, anti GTD antibody, anti HHG antibody, anti NROB1 antibody, anti dosage-sensitive sex reversal antibody, anti gonadotropin deficiency antibody, anti nuclear hormone receptor antibody

orb19492 (0.5ug/ml) staining of human testis lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Quick Database Links

Gene Symbol

DAX1 / NR0B1

NCBI Gene ID

190