Dachshund homolog 2/DACH2 Antibody

SKU: orb19775

Description

Goat polyclonal antibody to DACH2

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:64000, WB: 0.03-0.1 μg/ml
• Reactivity: Mouse
• Predicted Reactivity: Human
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Approx 65-70kDa band observed in lysates of cell line NIH3T3 (calculated MW of 65.3kDa according to human NP_444511.1 and of 68.6kDa according to mouse NP_291083.1). Recommended concentration: 0.03-0.1 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: Dachshund homolog 2 / DACH2
• Protein Sequence: TRKQAVNSSRPGR
• Molecular Weight: 65.3
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti DACH2 antibody, anti dachshund homolog 2 (Drosophila) antibody, anti RP11-345E19.1 antibody, anti FLJ31391 antibody, anti MGC138545 antibody, anti dachshund 2 antibody

orb19775 (0.03ug/ml) staining of NIH3T3 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Quick Database Links

Gene Symbol

Dachshund homolog 2 / DACH2

NCBI Gene ID

117154