Dachshund homolog 1 Antibody

• Catalog Number: orb19965
• Category: Antibodies
• Description: Goat polyclonal antibody to DACH1
• Target: Dachshund homolog 1
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Canine, Mouse, Porcine, Rat
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: SPVENTPQNNECK
• RRID: AB_10749255
• MW: 73.0; 57.3; 51.9
• Tested applications: ELISA, WB
• Dilution range: ELISA: 1:16000, WB: 0.1-0.3 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx 75 and 52kDa bands observed in Human Kidney lysates (calculated MW of 73.0kDa according to NP_542937.1 and of 51.9kDa according to NP_004383.2). Recommended concentration: 0.1-0.3 μg/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti OTTHUMP00000040839 antibody, anti dachshund h
• Research Area: Developmental Biology, Epigenetics
• Note: For research use only
• Entrez: 1602

orb19965 (0.1ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.