Cystatin 8 (aa 23-34) Antibody

SKU: orb20439

Description

Goat polyclonal to Cystatin 8

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:64000, WB: 0.1-0.3 μg/ml
• Predicted Reactivity: Human
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: In transfected HEK293 transiently expressing Human CST8 (myc and DYKDDDDK tagged), a band of approx. 23kDa is observed. No bands are observed in mock-transfected HEK293 and the same band is observed using anti-myc tag antibody. Recommended concentration, 0.1-0.3 μg/ml.Experiment Notes: Purchase the +ve control! The transfected lysate used in QC is available for sale.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: Cystatin 8 (aa 23-34)
• Protein Sequence: DPKKNETGVLRK
• Molecular Weight: 16.3
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti CST8 antibody, anti cystatin 8 (cystatin-related epididymal specific) antibody, anti CRES antibody, anti cystatin 8 antibody, anti cystatin-related epididymal spermatogenic protein antibody, anti cystatin-related epididymal-specific antibody

HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human CST8 (RC210130) with C-terminal MYC tag probed with orb20439 (1ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK294 probed with orb20601 (1mg/ml) in Lane

Quick Database Links

Gene Symbol

Cystatin 8 (aa 23-34)

NCBI Gene ID

10047