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Item 1 of 2
Goat anti-CYBB / GP91-PHOX Antibody
Catalog Number: orb20254
| Catalog Number | orb20254 |
|---|---|
| Category | Antibodies |
| Description | Goat polyclonal to CYBB |
| Species/Host | Goat |
| Clonality | Polyclonal |
| Tested applications | ELISA, IHC |
| Reactivity | Canine, Human |
| Dilution range | ELISA: 1:64000, IHC-P: 1-2µg/ml |
| Conjugation | Unconjugated |
| MW | 65.3 |
| Target | CYBB / GP91-PHOX |
| Entrez | 1536 |
| Protein Sequence | SYLNFARKRIKNP |
| RRID | AB_10748822 |
| Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
| Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Alternative names | anti CYBB antibody, anti cytochrome b-245, beta po Read more... |
| Note | For research use only |
| Application notes | ELISA: Peptide ELISA: antibody detection limit dilution 1:2000.WB: Approx 85Da band observed in Human Peripheral Blood Mononucleocytes ysates (calculated MW of 65.3kDa according to NP_000388.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Teahan et al, Nature. 1987 Jun 25-Jul 1;327(6124):720-1. PMID: 3600769). Recommended concentration: 0.03-0.1 μg/ml. |
| Expiration Date | 12 months from date of receipt. |
Immunohistochemical analysis of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining with CYBB antibody.
orb20254 (0.03ug/ml) staining of Human Peripheral Blood Mononucleocytes lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
