CTGF Antibody

• Catalog Number: orb421982
• Category: Antibodies
• Description: Goat polyclonal antibody to CTGF
• Target: CTGF
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Canine, Porcine
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: TLPVEFKCPDGE
• MW: 38.1
• Tested applications: ELISA, IF, IHC
• Dilution range: ELISA: 1:64000, IHC-P: 10ug/ml
• Application notes: IHC: Paraffin embedded Rat Liver. Recommended concentration: 6-8µg/ml.. Notes: Immunofluorescence: Strong expression of the protein seen in the cytoplasm/membrane of NIH3T3 and HepG2 cells. Recommended concentration: 10µg/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: CTGF; connective tissue growth factor; CCN2; HCS24
• Note: For research use only
• Entrez: 1490
• Expiration Date: 12 months from date of receipt.

Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml), showing cytoplasmic/membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml).

Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml), showing cytoplasmic/membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml).

6 μg/ml staining of paraffin embedded Rat Liver. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

Negative Control showing staining of paraffin embedded Rat Liver, with no primary antibody.