CSN2/TRIP15 Antibody

SKU: orb18512

Description

Goat polyclonal antibody to CSN2

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:16000, WB: 1-3 μg/ml, IHC-P: 3-5 μg/ml
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Canine, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.IHC: In paraffin embedded Human Thyroid Gland shows nclear and cytoplasm staining in activated epithelial cells Recommended concentration, 3-5ug/ml.WB: Approx. 48kDa band observed in Human Brain, Mouse Brain lysates and in lysates of Mouse putuitary cell line NIH3T3 (calculated MW of 51.6kDa according to NP_004227.1). Recommended concentration: 1-3 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: CSN2 / TRIP15
• Molecular Weight: 51.6; 52.4
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Disclaimer: For research use only

Alternative Names

anti CASB antibody, anti CSN 2 antibody, anti Csn2 antibody

Immunohistochemical analysis of formalin-fixed and paraffin embedded Human Thyroid Gland shows nclear and cytoplasm (3-5ug/ml) using CSN2 antibody

Western blot analysis of NIH-3T3 lysates (0.03ug/ml) using CSN2 antibody

Quick Database Links

Gene Symbol

CSN2 / TRIP15

NCBI Gene ID

9318

References

Gao, H. N. et al. Combination of histidine, lysine, methionine, and leucine promotes β-casein synthesis via the mechanistic target of rapamycin signaling pathway in bovine mammary epithelial cells J Dairy Sci, 100, 7696-7709 (2017)

PubMed: 28647331

Applications

WB

Reactivity

Bovine

Luo, Chaochao et al. Sestrin2 Negatively Regulates Casein Synthesis through the SH3BP4-mTORC1 Pathway in Response to AA Depletion or Supplementation in Cow Mammary Epithelial Cells J. Agric. Food Chem., (2019)

PubMed: 30969118

Applications

WB

Reactivity

Bovine

Jinyu Zhou 1 2 3, Shuai Lian 1 2 3, Zijian Geng 1 2 3, Yuejie Yang 4 5 6, Rui Wu 7, Jianfa Wang 8 9 10 Lactiplantibacillus plantarum promotes lactoferrin synthesis and secretion in bovine mammary epithelial cells through STAT3 and AP-1 transcription factor pathways In Vitro Cell Dev Biol Anim, (2025)

PubMed: 40802133

Applications

WB

Reactivity

Bovine

Dai, W. T. et al. Seryl-tRNA synthetase-mediated essential amino acids regulate β-casein synthesis via cell proliferation and mammalian target of rapamycin (mTOR) signaling pathway in bovine mammary epithelial cells J Dairy Sci, 101, 10456-10468 (2018)

PubMed: 30219419

Applications

WB

Reactivity

Bovine