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Item 1 of 2
| Catalog Number | orb18512 |
|---|---|
| Category | Antibodies |
| Description | Goat polyclonal antibody to CSN2 |
| Species/Host | Goat |
| Clonality | Polyclonal |
| Tested applications | ELISA, IHC, WB |
| Reactivity | Bovine, Canine, Human, Mouse, Rat |
| Dilution range | ELISA: 1:16000, WB: 1-3 μg/ml, IHC-P: 3-5 μg/ml |
| Conjugation | Unconjugated |
| MW | 51.6; 52.4 |
| Target | CSN2 / TRIP15 |
| Entrez | 9318 |
| Protein Sequence | LNSLNQAVVSKLA |
| RRID | AB_10750968 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Alternative names | anti CASB antibody, anti CSN 2 antibody, anti Csn2 Read more... |
| Note | For research use only |
| Application notes | ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.IHC: In paraffin embedded Human Thyroid Gland shows nclear and cytoplasm staining in activated epithelial cells Recommended concentration, 3-5ug/ml.WB: Approx. 48kDa band observed in Human Brain, Mouse Brain lysates and in lysates of Mouse putuitary cell line NIH3T3 (calculated MW of 51.6kDa according to NP_004227.1). Recommended concentration: 1-3 μg/ml. |
| Expiration Date | 12 months from date of receipt. |
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Gao, H. N. et al. Combination of histidine, lysine, methionine, and leucine promotes β-casein synthesis via the mechanistic target of rapamycin signaling pathway in bovine mammary epithelial cells J Dairy Sci, 100, 7696-7709 (2017)
Applications
WB
Reactivity
Bovine
Luo, Chaochao et al. Sestrin2 Negatively Regulates Casein Synthesis through the SH3BP4-mTORC1 Pathway in Response to AA Depletion or Supplementation in Cow Mammary Epithelial Cells J. Agric. Food Chem., (2019)
Applications
WB
Reactivity
Bovine
Dai, W. T. et al. Seryl-tRNA synthetase-mediated essential amino acids regulate β-casein synthesis via cell proliferation and mammalian target of rapamycin (mTOR) signaling pathway in bovine mammary epithelial cells J Dairy Sci, 101, 10456-10468 (2018)
Applications
WB
Reactivity
Bovine
Immunohistochemical analysis of formalin-fixed and paraffin embedded Human Thyroid Gland shows nclear and cytoplasm (3-5ug/ml) using CSN2 antibody
Western blot analysis of NIH-3T3 lysates (0.03ug/ml) using CSN2 antibody
