CRTC2 Antibody

SKU: orb22556

Description

mTORC2 antibody

Research Area

Neuroscience

Images & Validation

• Tested Applications: ELISA, FC, IF, WB
• Dilution Range: ELISA: 1:64000, WB: 1-3 μg/ml, IHC-P: 10ug/ml
• Reactivity: Human, Rat
• Predicted Reactivity: Bovine, Canine, Mouse, Porcine
• Application Notes:
  WB: Approx 90kDa band observed in lysates of cell line Jurkat (calculated MW of 73.2kDa according to NP_083157.1). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 1-3 ug/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: CRTC2
• Protein Sequence: DPAVEDSFRSDRLQ
• Molecular Weight: 73.2
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti--transducer of regulated cAMP response element-binding protein 2 antibody, anti-OTTMUSP00000023505 antibody, anti-Torc2 antibody, anti-4632407F12Rik antibody, anti-Crtc2 antibody

Western blot analysis of Jurkat cell lysate using mTORC2 antibody

Quick Database Links

Gene Symbol

CRTC2

NCBI Gene ID

200186