Crot (rat) (aa178-199) Antibody

SKU: orb107646

Description

Goat polyclonal to Crot

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:32000, WB: 1-3 μg/ml, IHC-P: 5 μg/ml
• Reactivity: Human, Mouse
• Predicted Reactivity: Canine, Rat
• Application Notes:
  WB: Approx 70kDa band observed in Mouse Liver lysates (calculated MW of 70.3kDa according to NP_114193.1). Recommended concentration: 1-3µg/ml. Primary incubation was 1 hour.. IHC: Paraffin embedded Human Brain (Cortex) and Liver. Recommended concentration: 5µg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: Crot (rat) (aa178-199)
• Protein Sequence: NYFKTESEGHCPTH
• Molecular Weight: 70.3
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti Crot antibody

Western blot analysis of Mouse Liver lysate (35ug protein in RIPA buffer) using Crot antibody

Immunohistochemical staining of Human Cortex using Crot antibody

Immunohistochemical staining of Human Liver using Crot antibody

Quick Database Links

Gene Symbol

Crot (rat) (aa178-199)