Crot (rat) (aa178-199) Antibody

• Catalog Number: orb107646
• Category: Antibodies
• Description: Goat polyclonal to Crot
• Target: Crot (rat) (aa178-199)
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Predicted Reactivity: Canine, Rat
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: NYFKTESEGHCPTH
• MW: 70.3
• Tested applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:32000, WB: 1-3 μg/ml, IHC-P: 5 μg/ml
• Application notes: WB: Approx 70kDa band observed in Mouse Liver lysates (calculated MW of 70.3kDa according to NP_114193.1). Recommended concentration: 1-3µg/ml. Primary incubation was 1 hour.. IHC: Paraffin embedded Human Brain (Cortex) and Liver. Recommended concentration: 5µg/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti Crot antibody
• Note: For research use only

Western blot analysis of Mouse Liver lysate (35ug protein in RIPA buffer) using Crot antibody

Immunohistochemical staining of Human Cortex using Crot antibody

Immunohistochemical staining of Human Liver using Crot antibody