Goat anti-CRHR1 / CRF-R (aa 107 - 117) Antibody

SKU: orb19649

Description

Goat polyclonal antibody to CRHR1

Research Area

Epigenetics, GPCR, Neuroscience, Signaling Pathways

Images & Validation

• Tested Applications: ELISA, IF, WB
• Dilution Range: ELISA: 1:128000, WB: 0.1-0.3 μg/ml, IHC-P: 10 μg/ml
• Reactivity: Human, Mouse, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.IHC: This product was successfully used on cryosection of mouse brain (PMID: 22570383)WB: Approx 60-70kDa band observed in human brain (cerebellum) and human colon lysates (calculated MW of 47.7kDa according to NP_004373.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Xu et al, Mol Pharmacol. 2001 Mar;59(3):485-92; PMID: 11179443). Recommended concentration: 0.1-0.3 μg/ml.Experiment Notes: Immunofluorescence: This product was successfully used in immunofluorescence on mouse brain at 2ug/ml when incubated at 4C for 40h.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: CRHR1 / CRF-R (aa 107 - 117)
• Protein Sequence: NEEKKSKVHYH
• Molecular Weight: 47.7
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti CRHR1 antibody, anti CRF-R antibody, anti corticotropin releasing hormone receptor 1 antibody, anti CRF1 antibody, anti CRFR1 antibody, anti CRH-R1h antibody, anti CRHR antibody, anti CRHR1f antibody, anti corticotropin releasing hormone receptor variant 1h antibody, anti seven transmembrane helix receptor antibody

2 μg/ml staining of Human Duodenum lysate (35 μg protein in RIPA buffer). Detected by chemiluminescence.

Quick Database Links

Gene Symbol

CRHR1 / CRF-R (aa 107 - 117)

NCBI Gene ID

1394