Goat anti-CRHR1 / CRF-R (aa 107 - 117) Antibody

• Catalog Number: orb19649
• Category: Antibodies
• Description: Goat polyclonal antibody to CRHR1
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IF, WB
• Reactivity: Human, Mouse, Rat
• Dilution range: ELISA: 1:128000, WB: 0.1-0.3 μg/ml, IHC-P: 10 μg/ml
• Conjugation: Unconjugated
• MW: 47.7
• Target: CRHR1 / CRF-R (aa 107 - 117)
• Entrez: 1394
• Protein Sequence: NEEKKSKVHYH
• RRID: AB_10749926
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti CRHR1 antibody, anti CRF-R antibody, anti cor
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.IHC: This product was successfully used on cryosection of mouse brain (PMID: 22570383)WB: Approx 60-70kDa band observed in human brain (cerebellum) and human colon lysates (calculated MW of 47.7kDa according to NP_004373.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Xu et al, Mol Pharmacol. 2001 Mar;59(3):485-92; PMID: 11179443). Recommended concentration: 0.1-0.3 μg/ml.Experiment Notes: Immunofluorescence: This product was successfully used in immunofluorescence on mouse brain at 2ug/ml when incubated at 4C for 40h.
• Expiration Date: 12 months from date of receipt.

2 μg/ml staining of Human Duodenum lysate (35 μg protein in RIPA buffer). Detected by chemiluminescence.