Goat anti-CREB1 (aa96-109) Antibody

• Catalog Number: orb137115
• Category: Antibodies
• Description: Goat polyclonal antibody to CREB1
• Target: CREB1 (aa96-109)
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Bovine, Canine, Human, Mouse, Porcine
• Concentration: < p>0.1-0.3 μg/ml.

• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: DSQESVDSVTDSQK
• MW: 35.1; 36.7
• Tested applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:128000, WB: 0.1-0.3 μg/ml, IHC-P: 2.5 μg/ml
• Application notes: WB: Approx 45kDa band observed in nuclear lysates of cell lines Jurkat and NIH3T3 (calculated MW of 36.7kDa according to NP_604391.1). This molecular weight is routinely observed by other sources. Recommended concentration: 0.1-0.3µg/ml. Primary incubation wa. IHC: Paraffin embedded Human Prostate. Recommended concentration: 2.5µg/ml.. Notes: Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti CREB1 antibody, anti cAMP responsive element
• Note: For research use only
• Entrez: 1385

Western blot analysis of Jurkat (Lane 1) and NIH3T3 (Lane 2) nuclear lysates using CREB1 antibody

Immunohistochemical staining of Human Prostate using CREB1 antibody