CPLA2-zeta Antibody

SKU: orb20598

Description

Goat polyclonal to CPLA2-zeta

Research Area

Cardiovascular Disease

Images & Validation

• Tested Applications: ELISA
• Dilution Range: ELISA: 1:16000, WB: 1 μg/ml
• Predicted Reactivity: Bovine, Human, Mouse, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Preliminary experiments gave bands at approx 55-60kDa and 35kDa in Human Placenta lysates after 1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 95.1kDa according to NP_998765.3. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: CPLA2-zeta
• Protein Sequence: QTAEEKAFGDFVINR
• Molecular Weight: 95.1
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti PLA2G4F antibody, anti phospholipase A2, group IVF antibody, anti DKFZp666G192 antibody, anti DKFZp781B229 antibody, anti PLA2G4FZ antibody, anti cytosolic phospholipase A2 zeta antibody, anti cPLA2-zeta antibody

Quick Database Links

Gene Symbol

CPLA2-zeta

NCBI Gene ID

255189