Cortactin/EMS1 Antibody

SKU: orb18335

Description

Goat polyclonal antibody to CTTN

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:32000, WB: 0.01-0.03 μg/ml
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Canine
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx. 80kDa band observed in Mouse and Rat Brain lysates (calculated MW of 61.6kDa according to Human NP_005222.2 and of 61.2kDa according to Mouse NP_031829.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Paradis et al, J Cell Sci. 2008 Jun 15;121(Pt 12):1965-72; PMID 18495841). Recommended concentration: 0.01-0.03 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: Cortactin / EMS1
• Protein Sequence: WKASAGHAVSIA
• Molecular Weight: 61.6; 57.5
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti EMS1 antibody, anti CORTACTIN antibody, anti ems1 sequence (mammary tumor and squamous cell carcinoma-associated (p80/85 src substrate) antibody, anti Cttn antibody, anti oncogene EMS1 antibody, anti CTTN antibody, anti FLJ34459 antibody, anti 1110020L01Rik antibody

Western blot analysis of Mouse Brain lysate using CTTN antibody

Western blot analysis of HEK293 cell lysate using CTTN antibody

Western blot analysis of Human Brain (Cerebellum) lysate using CTTN antibody

Quick Database Links

Gene Symbol

Cortactin / EMS1

NCBI Gene ID

2017