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Item 1 of 6
Item 1 of 6
Goat anti-Connexin 43 / GJA1 Antibody
Catalog Number: orb20237
| Catalog Number | orb20237 |
|---|---|
| Category | Antibodies |
| Description | Goat polyclonal antibody to GJA1 |
| Species/Host | Goat |
| Clonality | Polyclonal |
| Tested applications | ELISA, FC, IF, WB |
| Reactivity | Canine, Human, Porcine, Rat |
| Dilution range | ELISA: 1:32000, WB: 0.1-1 μg/ml |
| Conjugation | Unconjugated |
| MW | 43 |
| Target | Connexin 43 / GJA1 |
| Entrez | 2697 |
| Protein Sequence | QPFDFPDDNQNSKK |
| RRID | AB_10749160 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Alternative names | anti GJA1 antibody, anti gap junction protein, alp Read more... |
| Note | For research use only |
| Application notes | ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Approx 40kDa band observed in Rat Brain lysates (calculated MW of 43.0kDa according to Human NP_000156.1 and to Rat NP_036699.1). Recommended concentration: 0.3-1 μg/ml. |
| Expiration Date | 12 months from date of receipt. |
Western blot analysis of Human and Rat Heart lysate using GJA1 antibody
Western blot analysis of Human and Rat Heart lysate using GJA1 antibody
0.1 µg/mL staining of Human Cerebellum lysate (35 µg protein in RIPA buffer). Detected by chemiluminescence.
Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1 hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing cytoplasmic and plasma membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).
Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1 hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing cytoplasmic, plasma membrane and nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).
Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1 hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (1 µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
