Goat anti-Complement factor H Antibody

• Catalog Number: orb19049
• Category: Antibodies
• Description: Goat polyclonal antibody to CFH
• Target: Complement factor H
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: HLVPDRKKDQYK
• RRID: AB_10750342
• MW: 139
• Tested applications: ELISA, FC, IHC, WB
• Dilution range: ELISA: 1:32000, WB: 0.03-0.3 μg/ml, IHC-P: 5 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 170kDa band observed in human lung lysates (calculated MW of 139kDa according to NP_000177.2). Recommended concentration: 0.03-0.1 μg/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti complement factor H antibody, anti HGNC:4883
• Research Area: Cardiovascular Disease, Epigenetics, Immunology, I
• Note: For research use only
• Entrez: 3075
• Expiration Date: 12 months from date of receipt.

orb19049 staining (0.03ug/ml) of human lung lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

orb19049 (5µg/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.