Goat anti-COL4A6 Antibody

• Catalog Number: orb20338
• Category: Antibodies
• Description: Goat polyclonal antibody to Collagen IV
• Target: COL4A6
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: PSPEFETETLHNKES
• RRID: AB_10751488
• MW: 164; 157; 161;158
• Tested applications: ELISA, FC, IF
• Dilution range: ELISA: 1:32000, IHC-P: 10ug/ml
• Application notes: This antibody is expected to recognize both reported isoforms (NP_001838.2; NP_378667.1).ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Preliminary experiments gave an approx 20kDa band in Human Heart, Kidney and Skeletal Muscle lysates after 0.3 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 164kDa according to NP_001838.2 and NP_378667.1. The 20kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: COL4A6; collagen, type IV, alpha 6; MGC88184; OTTH
• Research Area: Epigenetics
• Note: For research use only
• Entrez: 1288