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| Catalog Number | orb176711 |
|---|---|
| Category | Antibodies |
| Description | Goat polyclonal antibody to Collagen IV |
| Target | COL4A3 (aa341-353) |
| Clonality | Polyclonal |
| Species/Host | Goat |
| Conjugation | Unconjugated |
| Reactivity | Human |
| Predicted Reactivity | Bovine, Canine |
| Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Protein Sequence | RGPTEYYDTYQEK |
| MW | 162 |
| Tested applications | ELISA, IHC |
| Dilution range | ELISA: 1:32000, IHC-P: 4-6µg/ml |
| Application notes | IHC: In paraffin embedded Human Kidney shows staining at the luminar side of the epithelial cell membranes. Recommended concentration, 4-6µg/ml. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | COL4A3; collagen, type IV, alpha 3 (Goodpasture an Read more... |
| Research Area | Epigenetics |
| Note | For research use only |
| Entrez | 1285 |
| Expiration Date | 12 months from date of receipt. |

Immunohistochemical staining of Human Kidney using Collagen IV antibody