Goat anti-COG1 Antibody

SKU: orb20060

Description

Goat polyclonal antibody to COG1

Research Area

Epigenetics

Images & Validation

• Tested Applications: ELISA, IF, WB
• Dilution range: ELISA: 1:64000, WB: 0.1-0.3 μg/ml
• Reactivity: Human
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Approx. 110kDa band observed in Human Brain (Cerebellum, Amygdala) lysates (calculated MW of 109kDa according to NP_061184.1). Recommended concentration: 0.1-0.3 μg/ml. An additional band of unknown identity was also consistently observed at 45kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: COG1
• Molecular Weight: 109
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Disclaimer: For research use only

Alternative Names

anti COG1 antibody, anti component of oligomeric golgi complex 1 antibody, anti CDG2G antibody, anti DKFZp762L1710 antibody, anti KIAA1381 antibody, anti LDLB antibody, anti conserved oligomeric Golgi complex protein 1 antibody, anti low density lipoprotein receptor defect B complementing antibody

orb20060 (0.1ug/ml) staining of Human Cerebellum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Quick Database Links

Gene Symbol

COG1

NCBI Gene ID

9382