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| Catalog Number | orb125014 |
|---|---|
| Category | Antibodies |
| Description | Goat polyclonal antibody to CMA1 |
| Target | CMA1 |
| Clonality | Polyclonal |
| Species/Host | Goat |
| Conjugation | Unconjugated |
| Reactivity | Human |
| Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Protein Sequence | QFRHPKYNTSTLHHD |
| MW | 27.3 |
| Tested applications | ELISA, IHC, WB |
| Dilution range | ELISA: 1:128000, WB: 0.5-1 μg/ml, IHC-P: 1.5 μg/ml |
| Application notes | WB: In transfected HEK293 transiently expressing full-length Human CMA1 (myc and DYKDDDDK tagged), a band of approx. 35kDa was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-DYKDDDDK tag antibody. Recomm. IHC: Paraffin embedded Human Testis and Tonsil (Mast cells). Recommended concentration: 1.5µg/ml.. Notes: Purchase the +ve control! The transfected lysate used to QC this product is available for sale. Please email [email protected] to request datasheet, pricing and delivery information. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | anti Alpha-chymase antibody, anti Chymase 1 antibo Read more... |
| Note | For research use only |
| Entrez | 1215 |

Western blot analysis of HEK293 lysate using Mast Cell Chymase antibody

Immunohistochemical staining of Human Skin using Mast Cell Chymase antibody
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