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Goat anti-CLPB Antibody
Catalog Number: orb19558
| Catalog Number | orb19558 |
|---|---|
| Category | Antibodies |
| Description | Goat polyclonal antibody to CLPB |
| Target | CLPB |
| Clonality | Polyclonal |
| Species/Host | Goat |
| Conjugation | Unconjugated |
| Reactivity | Human |
| Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Protein Sequence | DKDSKTRRLDIRAP |
| RRID | AB_10762482 |
| MW | 78.7 |
| Tested applications | ELISA |
| Dilution range | ELISA: 1:32000, WB: 0.1 μg/ml |
| Application notes | ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Preliminary experiments gave bands at approx 50kDa and 48kDa in lysates of HeLa and HepG2 after 0.1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 78.7kDa according to NP_110440.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported |
| Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
| Alternative names | anti CLPB antibody, anti ClpB caseinolytic peptida Read more... |
| Note | For research use only |
| Entrez | 81570 |
