CLIC4 Antibody

SKU: orb19557

Description

Goat polyclonal antibody to CLIC4

Research Area

Epigenetics

Images & Validation

• Tested Applications: ELISA, IF, IHC, WB
• Dilution range: ELISA: 1:64000, WB: 0.1-0.3 μg/ml, IHC-P: 5 μg/ml
• Reactivity: Human, Mouse
• Predicted Reactivity: Canine, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Approx 30kDa band observed in human kidney and human placenta lysates (calculated MW of 28.8kDa according to NP_039234.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Edwards et al, Am J Physiol. 1999 Mar;276(3 Pt 2):F398-408.; PMID: 10070163). Recommended concentration: 0.1-0.3 μg/ml.Experiment Notes: Immunofluorescence: Min6 cells transiently expressing mouse Clic4 shows cytoplasmic staining of two cells. Recommended concentration, 5ug/ml with overnight incubation.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: CLIC4
• Molecular Weight: 28.8
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Disclaimer: For research use only

Alternative Names

anti CLIC4 antibody, anti chloride intracellular channel 4 antibody, anti CLIC4L antibody, anti DKFZP566G223 antibody, anti FLJ38640 antibody, anti H1 antibody, anti huH1 antibody, anti p64H1 antibody, anti chloride intracellular channel 4 like antibody

Western blot analysis of human kidney lysate (0.1ug/ml) using CLIC4 antibody

Confocal immunofluoroscence analysis of min6 cells using CLIC4 antibody

Immunohistochemical staining of Human Heart using CLIC4 antibody

Quick Database Links

Gene Symbol

CLIC4

NCBI Gene ID

25932