CKB/Brain Creatine Kinase Antibody

SKU: orb19213

Description

Goat polyclonal antibody to CKB

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:2000, WB: 0.1-0.3 μg/ml
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Porcine, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:2000.WB: Approx 45-48kDa band observed in human brain lysates (calculated MW of 42.6kDa according to NP_001814.2). Recommended concentration: 0.1-0.3 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: CKB / Brain Creatine Kinase
• Protein Sequence: KPSDEHKTDLNPDN
• Molecular Weight: 42.6
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti CKB antibody, anti brain creatine kinase antibody, anti creatine kinase, brain antibody, anti B-CK antibody, anti CKBB antibody, anti creatine kinase B-chain antibody, anti creatine kinase-B antibody

orb19213 (0.1ug/ml) staining of human brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Quick Database Links

Gene Symbol

CKB / Brain Creatine Kinase

NCBI Gene ID

1152