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CHRNB4 Antibody

SKU: orb19811

Description

Goat polyclonal antibody to CHRNB4

Images & Validation

Tested ApplicationsELISA, IHC
Dilution rangeELISA: 1:16000, WB: 0.2 μg/ml, IHC-P: 2.5 μg/ml
ReactivityHuman
Predicted ReactivityBovine
Application Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Preliminary experiments gave an approx 40kDa band in human testis lysates after 0.2 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 56.4kDa according to NP_000741.1. The 40kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Key Properties

HostGoat
ClonalityPolyclonal
TargetCHRNB4
Molecular Weight56.4
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
DisclaimerFor research use only

Alternative Names

anti CHRNB4 antibody, anti cholinergic receptor antibody, anti nicotinicN: beta 4 antibody, anti Cholinergic receptor, neuronal nicotinic, beta polypeptide-4 antibody, anti cholinergic receptor, nicotinic, beta polypeptide 4 antibody, anti neuronal nicotinic receptor beta 4 subunit antibody

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NCBI Gene Details

No NCBI Gene data available

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Protocol Information

IHC
Immunohistochemistry
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

CHRNB4 Antibody (orb19811)

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Available Sizes

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100 μg
$ 500.00
DispatchUsually dispatched within 5-10 working days
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