CHRNB4 Antibody

SKU: orb19811

Description

Goat polyclonal antibody to CHRNB4

Research Area

Epigenetics

Images & Validation

• Tested Applications: ELISA, IHC
• Dilution Range: ELISA: 1:16000, WB: 0.2 μg/ml, IHC-P: 2.5 μg/ml
• Reactivity: Human
• Predicted Reactivity: Bovine
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Preliminary experiments gave an approx 40kDa band in human testis lysates after 0.2 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 56.4kDa according to NP_000741.1. The 40kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: CHRNB4
• Protein Sequence: DYRLTWNSSRYEGVN
• Molecular Weight: 56.4
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti CHRNB4 antibody, anti cholinergic receptor antibody, anti nicotinicN: beta 4 antibody, anti Cholinergic receptor, neuronal nicotinic, beta polypeptide-4 antibody, anti cholinergic receptor, nicotinic, beta polypeptide 4 antibody, anti neuronal nicotinic receptor beta 4 subunit antibody

Quick Database Links

Gene Symbol

CHRNB4

NCBI Gene ID

1143