CHRNA4 (aa29-43) Antibody

SKU: orb19976

Description

Goat polyclonal antibody to CHRNA4

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:64000, WB: 1-3 μg/ml
• Reactivity: Human, Rat
• Predicted Reactivity: Bovine, Canine, Mouse
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Approx 70kDa band observed in Rat Brain lysates (calculated MW of 70.0kDa according to Human NP_000735.1and of 70.2kDa according to Rat NP_077330.1). Recommended concentration: 1-3 μg/ml. An additional band of unknown identity was also consistently observed at 22kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: CHRNA4 (aa29-43)
• Protein Sequence: HVETRAHAEERLLKK
• Molecular Weight: 70
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti CHRNA4 antibody, anti cholinergic receptor, nicotinic, alpha 4 antibody, anti BFNC antibody, anti EBN antibody, anti EBN1 antibody, anti NACRA4 antibody, anti cholinergic receptor, nicotinic, alpha 4 subunit antibody, anti cholinergic receptor, nicotinic, alpha polypeptide 4 antibody, anti neuronal acetylcholine receptor alpha 4 subunit antibody, anti neuronal nicotinic acetylcholine receptor alpha-4 subunit antibody

orb19976 (0.3ug/ml) staining of Rat Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Quick Database Links

Gene Symbol

CHRNA4 (aa29-43)

NCBI Gene ID

1137