Goat Anti-Chicken IgY (Fc) Antibody (HRP)

• Catalog Number: orb1236934
• Category: Antibodies
• Description: Goat Anti-Chicken IgY (Fc) Antibody (HRP)
• Species/Host: Goat
• Tested applications: ELISA, WB
• Reactivity: Gallus
• Isotype: IgY
• Immunogen: Chicken IgY Fc fragment
• Dilution range: The affinity purified antibodies react only with chicken IgY and not IgM or IgA immunoglobulins by immunodiffusion and IEP techniques. In a sandwich ELISA against 1 μg/mL chicken IgY, conjugated antibody diluted to 1:100,000 produced an O.D. = 1.0 in a 15 minute reaction with TMB substrate. Recommended Western Blot dilution: 1:2,000 – 1:4,000 when used for colorimetric assay, and 1:10,000 or up for ECL detection system. Optimal conjugate working dilutions should be determined for your particular assay conditions.
• Form/Appearance: Liquid
• Conjugation: HRP
• Storage: 4 °C, avoid frequent freeze-thaw
• Buffer/Preservatives: HEPES
• Note: For research use only
• Application notes: The affinity purified antibodies react only with chicken IgY and not IgM or IgA immunoglobulins by immunodiffusion and IEP techniques. In a sandwich ELISA against 1 μg/mL chicken IgY, conjugated antibody diluted to 1:100,000 produced an O.D. = 1.0 in a 15 minute reaction with TMB substrate. Recommended Western Blot dilution: 1:2,000 – 1:4,000 when used for colorimetric assay, and 1:10,000 or up for ECL detection system. Optimal conjugate working dilutions should be determined for your particular assay conditions.
• Expiration Date: 12 months from date of receipt.