CHD7 Antibody

SKU: orb19652

Description

The CHD7 protein belongs to a family of proteins that are thought to play a role in the organization of chromatin. Chromatin is the complex of DNA and protein that packages DNA into chromosomes. The CHD7 protein regulates the activity (expression) of several other genes through a process known as chromatin remodeling. The structure of chromatin can be changed (remodeled) to alter how tightly DNA is packaged. When DNA is tightly packed, gene expression is lower than when DNA is loosely packed. Researchers are working to determine which genes the CHD7 protein regulates. CHD7(Chromohelicase/ATPase DNA-binding protein 7) is a 350 kDa member of the third subgroup in the SNF2/RAD54 helicase family of proteins. It is an ATP-dependent chromatin remodeling factor that also binds histones (H3 at Lys4) and influences transcription.

Research Area

Epigenetics & Chromatin

Images & Validation

• Tested Applications: ELISA, IHC
• Dilution Range: ELISA: 1:64000, WB: 1 μg/ml, IHC-P: 3.75 μg/ml
• Reactivity: Human
• Predicted Reactivity: Mouse, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Preliminary experiments gave bands at approx 250kDa and 48kDa in Rat Spinal Cord lysates after 1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 336kDa according to Human NP_060250.2 and 175kDa according to predicted Rat NP_001101376.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: CHD7
• Protein Sequence: KDEIDEFANSPSE
• Molecular Weight: 336
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti-chromodomain helicase DNA binding protein 7 antibody, anti-FLJ20357 antibody, anti-FLJ20361 antibody, anti-IS3 antibody, anti-KIAA1416 antibody, anti-Scoliosis, idiopathic 3 antibody

Immunohistochemical staining of Human Cortex using CHD7 antibody.

Quick Database Links

Gene Symbol

CHD7

NCBI Gene ID

55636