Goat anti-CHD5 Antibody

• Catalog Number: orb19466
• Category: Antibodies
• Description: Goat polyclonal antibody to CHD5
• Target: CHD5
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Canine, Human, Mouse, Rat
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: QSRRQLKSDRDKP
• RRID: AB_10749372
• MW: 223
• Tested applications: ELISA, FC, IF
• Dilution range: ELISA: 1:64000, IF/ICC: 10μg/ml, FACS: 10ug/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Preliminary experiments gave an approx 90kDa band in Human Brain (Cerebellum and Frontal Cortex) and Adrenal Gland lysates after 1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 223kDa according to NP_056372.1. The 90kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti CHD5 antibody, anti chromodomain helicase DNA
• Note: For research use only
• Entrez: 26038

Immunofluorescence analysis of paraformaldehyde-fixed U2OS cells using CHD5 antibody.

Flow cytometric analysis of paraformaldehyde-fixed MCF7 cells using CHD5 antibody.