CHD5 Antibody

SKU: orb19466

Description

Goat polyclonal antibody to CHD5

Research Area

Cancer, Epigenetics

Images & Validation

• Tested Applications: ELISA, FC, IF
• Dilution Range: ELISA: 1:64000, IF/ICC: 10μg/ml, FACS: 10ug/ml
• Reactivity: Human
• Predicted Reactivity: Canine, Mouse, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Preliminary experiments gave an approx 90kDa band in Human Brain (Cerebellum and Frontal Cortex) and Adrenal Gland lysates after 1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 223kDa according to NP_056372.1. The 90kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: CHD5
• Protein Sequence: QSRRQLKSDRDKP
• Molecular Weight: 223
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti CHD5 antibody, anti chromodomain helicase DNA binding protein 5 antibody, anti DKFZp434N231 antibody, anti KIAA0444 antibody

Immunofluorescence analysis of paraformaldehyde-fixed U2OS cells using CHD5 antibody.

Flow cytometric analysis of paraformaldehyde-fixed MCF7 cells using CHD5 antibody.

Quick Database Links

Gene Symbol

CHD5

NCBI Gene ID

26038