Goat anti-CHCHD3 (aa151-164) Antibody

• Catalog Number: orb20495
• Category: Antibodies
• Description: Goat polyclonal antibody to CHCHD3
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Bovine, Canine, Human, Mouse, Rat, Sheep
• Dilution range: ELISA: 1:32000, WB: 0.3-1 μg/ml, IHC-P: 5 μg/ml
• Conjugation: Unconjugated
• MW: 26.2
• Target: CHCHD3 (aa151-164)
• Entrez: 54927
• Protein Sequence: RSSEFYRVTTEQYQ
• RRID: AB_10926751
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti coiled-coil-helix-coiled-coil-helix domain co
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 26.2kDa band observed in Human Placenta lysates (calculated MW of 26kDa according to NP_060282.1). Recommended concentration: 0.3-1 μg/ml.Anonymous customer reported ~30kDa in THP1 and in human primary monocyte derived dendritic cells.
• Expiration Date: 12 months from date of receipt.

orb20495 (0.3ug/ml) staining of Human Placenta lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb20495 (5µg/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.