CHA/TCFL5 Antibody

• Catalog Number: orb19348
• Category: Antibodies
• Description: Goat polyclonal antibody to TCFL5
• Target: CHA / TCFL5
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Canine, Mouse, Rat
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: KYIQERHGDSLKKE
• RRID: AB_10748767
• MW: 52.7
• Tested applications: ELISA, WB
• Dilution range: ELISA: 1:64000, WB: 0.3-1 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Approx 55kDa band observed in Human Testis lysates (calculated MW of 52.7kDa according to NP_006593.2). Recommended concentration: 0.3-1 μg/ml. An additional band of unknown identity was also consistently observed at 28kDa. This band was greatly reduced by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti TCFL5 antibody, anti CHA antibody, anti trans
• Research Area: Developmental Biology
• Note: For research use only
• Entrez: 10732

orb19348 (0.3ug/ml) staining of Human Testis lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.