Goat anti-CES1 Antibody

SKU: orb19790

Description

Goat polyclonal antibody to CES1

Research Area

Cell Biology

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:8000, WB: 0.03-0.1 μg/ml, IHC-P: 2-3µg/ml
• Reactivity: Human
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.IHC: In paraffin embedded Human Lung shows staining of lymphocytes in the vasculature.. Recommended concentration, 2-3ug/ml.WB: Approx 55-60kDa band observed in Human Liver and Ileum lysates (calculated MW of 62.6kDa according to NP_001020366.1). In transfected HEK293 transiently expressing CES1 a band of approx. 65kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.03-0.1 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: CES1
• Protein Sequence: NTQAAQKLKDKE
• Molecular Weight: 62.6; 62.5; 62.4
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti CES1 antibody, anti carboxylesterase 1 (monocyte/macrophage serine esterase 1) antibody, anti ACAT antibody, anti CEH antibody, anti CES2 antibody, anti HMSE antibody, anti HMSE1 antibody, anti MGC117365 antibody, anti PCE-1 antibody, anti SES1 antibody, anti TGH antibody, anti acyl coenzyme A:cholesterol acyltransferase antibody, anti carboxylesterase 1 antibody, anti carboxylesterase 2 (liver) antibody, anti cholesteryl ester hydrolase antibody, anti egasyn antibody, anti liver carboxylesterase antibody, anti triacylglycerol hydrolase antibody, anti monocyte/macrophage serine-esterase 1 antibody

orb19790 (0.03ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb19790 (2.5ug/ml) staining of paraffin embedded Human Lung. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Quick Database Links

Gene Symbol

CES1

NCBI Gene ID

1066