CENTG3/MRIP1 Antibody

• Catalog Number: orb18777
• Category: Antibodies
• Description: Goat polyclonal antibody to AGAP3
• Target: CENTG3 / MRIP1
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Mouse, Rat
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: NPSAELHRSPSLL
• RRID: AB_10751392
• MW: 98
• Tested applications: ELISA, IHC
• Dilution range: ELISA: 1:32000, WB: 1ug/ml, IHC-P: 5-10µg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.IHC: In paraffin embedded Human Kidney shows strong staining of brush borders in Proximal Convoluted tubules. Recommended concentration, 5-10ug/ml.WB: Preliminary experiments gave an approx 45kDa band in Human Brain and Human Liver lysates at 1 μg/ml. This band was successfully blocked by incubation with the immunising peptide. Please note that currently we cannot find an explanation in the literature for the band we observe given the predicted size of approx. 101kDda according to NP_114152. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti AGAP3 antibody, anti CENTG3 antibody, anti MR
• Note: For research use only
• Entrez: 116988

orb18777 (5ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.