CENTG3/MRIP1 Antibody

SKU: orb18777

Description

Goat polyclonal antibody to AGAP3

Images & Validation

• Tested Applications: ELISA, IHC
• Dilution Range: ELISA: 1:32000, WB: 1ug/ml, IHC-P: 5-10µg/ml
• Reactivity: Human
• Predicted Reactivity: Mouse, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.IHC: In paraffin embedded Human Kidney shows strong staining of brush borders in Proximal Convoluted tubules. Recommended concentration, 5-10ug/ml.WB: Preliminary experiments gave an approx 45kDa band in Human Brain and Human Liver lysates at 1 μg/ml. This band was successfully blocked by incubation with the immunising peptide. Please note that currently we cannot find an explanation in the literature for the band we observe given the predicted size of approx. 101kDda according to NP_114152. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: CENTG3 / MRIP1
• Protein Sequence: NPSAELHRSPSLL
• Molecular Weight: 98
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti AGAP3 antibody, anti CENTG3 antibody, anti MRIP1 antibody, anti centaurin, gamma 3 antibody, anti MRIP-1 antibody, anti MRIP-1 protein antibody, anti ArfGAP with GTPase domain, ankyrin repeat and PH domain 3 antibody, anti CRAG antibody, anti FLJ16146 antibody, anti FLJ34452 antibody, anti CRMP (collapsin response mediator protein) associated antibody, anti centaurin, gamma 3 antibody

orb18777 (5ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Quick Database Links

Gene Symbol

CENTG3 / MRIP1

NCBI Gene ID

116988