CENTB1/ACAP1 Antibody

• Catalog Number: orb18800
• Category: Antibodies
• Description: Goat polyclonal antibody to ACAP1
• Target: CENTB1 / ACAP1
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Rat
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: EKLSRRSHDLHTL
• RRID: AB_10755591
• MW: 81.5
• Tested applications: ELISA, WB
• Dilution range: ELISA: 1:32000, WB: 0.3-1 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 80-90kDa band observed in Jurkat lysates (predicted MW of 85kDa according to NP_055531). Recommended for use at 0.5-2 μg/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti ACAP1 antibody, anti CENTB1 antibody, anti ce
• Note: For research use only
• Entrez: 9744

orb18800 staining (0.5ug/ml) of Jurkat lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.