Goat anti-CDKN2A (isoform 1) / p16INK4a Antibody

• Catalog Number: orb19161
• Category: Antibodies
• Description: Goat polyclonal antibody against CDKN2A
• Target: CDKN2A (isoform 1) / p16INK4a
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: HARIDAAEGPSDIPD
• RRID: AB_10749106
• MW: 16.5; 17.9
• Tested applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:32000, WB: 0.1-1 μg/ml, IHC-P: 5 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx. 16-18kDa band observed in lysates of the cell lines A431 and HeLa (calculated MW of 16.5kDa according to NP_000068.1). Recommended concentration: 0.1-1 μg/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti-ARF antibody, anti-CCM2 antibody, anti-CDK4 i
• Research Area: Cancer, Epigenetics
• Note: For research use only
• Entrez: 1029
• Expiration Date: 12 months from date of receipt.

orb19161 (1ug/ml) staining of HeLa Lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb19161 (5µg/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.