Goat anti-CD274 / PD-L1 Antibody

SKU: orb18977

Description

Goat polyclonal antibody to CD274

Research Area

Epigenetics, Immunology

Images & Validation

• Tested Applications: ELISA, FC, IF, WB
• Dilution Range: ELISA: 1:128000, WB: 0.01-0.1 μg/ml, IF/ICC: 10μg/ml, FACS: 10ug/ml
• Reactivity: Human
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.IHC: In paraffin embedded Human Skel;etal Muscle shows staining of the striated muscular fibres. Recommended concentration, 4-6ug/ml. Data obtained with a previous batch.WB: Approx 35kDa band observed in Human Heart lysates (calculated MW of 33.3kDa according to NP_054862.1). Recommended concentration: 0.3-1.0 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: CD274 / PD-L1
• Protein Sequence: CKKQSDTHLEET
• Molecular Weight: 33.3; 20.2
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Anti-CD274 antibody, anti-Programmed cell death 1 ligand 1 antibody, anti-Programmed death ligand 1 antibody, anti-B7 homolog 1 antibody, anti-B7-H1 antibody.

Western blot analysis of Human Heart lysate using CD274 antibody

Flow cytometric analysis of paraformaldehyde fixed Jurkat cells using CD274 antibody

Immunofluorescence analysis of paraformaldehyde fixed U2OS cells using CD274 antibody

Immunofluorescence analysis of paraformaldehyde-fixed AA431 cells using CD274 antibody

Western blot analysis of U2OS cell lysate using CD274 antibody

Quick Database Links

Gene Symbol

CD274 / PD-L1

NCBI Gene ID

29126