CD19 (aa422-433) Antibody

SKU: orb107679

Description

Goat polyclonal to CD19

Research Area

Stem Cells

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:32000, WB: 1-3 μg/ml
• Reactivity: Human
• Predicted Reactivity: Bovine
• Application Notes:
  WB: Approx 75kDa band observed in lysates of cell lines Jurkat and MOLT4 (calculated MW of 61.1kDa according to NP_001761.3). The observed molecular weight corresponds to the glycosylated form. Recommended concentration: 1-3µg/ml. Primary incubation 1 hour at

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: CD19 (aa422-433)
• Protein Sequence: ENDSNLGQDQLS
• Molecular Weight: 61.2; 61.1
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti CD19 antibody

Western blot analysis of Jurkat lysate (35µg protein in RIPA buffer) using CD19 antibody

Quick Database Links

Gene Symbol

CD19 (aa422-433)

NCBI Gene ID

930