CD127/IL7R (mouse) Antibody

• Catalog Number: orb22503
• Category: Antibodies
• Description: Goat polyclonal antibody to IL7 Receptor
• Target: CD127 / IL7R (mouse)
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Predicted Reactivity: Mouse, Rat
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: DDHSFWCHSQLE
• RRID: AB_10934889
• MW: 51.6
• Tested applications: ELISA
• Dilution range: ELISA: 1:32000, WB: 2-4 μg/ml
• Application notes: WB: Approx 50kDa band observed in lysates of cell line NIH3T3 (calculated MW of 51.6kDa according to NP_032398.3). Recommended concentration: 2-4 μg/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti -MGC10755 antibody, anti-interleukin-7 recept
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

orb22503 (2ug/ml) staining of NIH3T3 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.