Goat Anti-CCR2 Antibody

• Catalog Number: orb2657253
• Category: Antibodies
• Description: Goat Anti-CCR2 Antibody
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Immunogen: Peptide with sequence RFIRNTNESGEEVT-C, from the N Terminus of the protein sequence according to NP_000638.1; NP_000639.1.
• Tested applications: ELISA, IF, IHC
• Dilution range: ELISA: 1:16000. IHC: Paraffin embedded Human Tonsil. Recommended concentration: 6µg/ml. Immunofluorescence: Strong expression of the protein seen in the membranes and cytoplasm of THP-1 cells. Recommended concentration: 10µg/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: chemokine (C-C motif) receptor 2, HGNC:1603, CC-CK
• Note: For research use only
• NCBI: NP_000638.1, NP_000639.1

Immunofluorescence analysis of paraformaldehyde fixed THP-1 cells immobilized on ShifixTM coverslip, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).

6 µg/ml staining of paraffin embedded Human Tonsil. Heat induced antigen retrieval with citrate buffer pH6, HRP-staining.

Negative Control showing staining of paraffin embedded Human Tonsil, with no primary antibody.