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CCL3 and CCL3L1 Antibody

SKU: orb19059

Description

Goat polyclonal antibody to CCL3L1; CCL3

Images & Validation

Tested ApplicationsELISA
Dilution rangeELISA: 1:32000, WB: 0.1 μg/ml
Predicted ReactivityHuman
Application Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Preliminary experiments gave bands at approx 26+28kDa (doublet) and 40kDa in Human Bone Marrow and Spleen lysates after 0.1 μg/ml antibody staining.The 26+28kDa doublet was also found in Human Lymph Node and in Tonsil lysates. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 10.2kDa according to NP_066286.1. The detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain these bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Key Properties

HostGoat
ClonalityPolyclonal
TargetCCL3 and CCL3L1
Molecular Weight10.1; 10.2
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
DisclaimerFor research use only

Alternative Names

anti CCL3 antibody, anti chemokine (C-C motif) ligand 3 antibody, anti G0S19-1 antibody, anti LD78ALPHA antibody, anti MIP-1-alpha antibody, anti MIP1A antibody, anti SCYA3 antibody, anti LD78 alpha beta antibody, anti Small inducible cytokine A3 antibody, anti small inducible cytokine A3 (homologous to mouse Mip-1a) antibody, anti CCL3L1 antibody, anti LD78 antibody, anti 464.2 antibody, anti SCYA3L antibody, anti G0S19-2 antibody, anti SCYA3L1 antibody, anti D17S1718 antibody, anti LD78BETA antibody, anti MGC12815 antibody, anti chemokine (C-C motif) ligand 3-like 1 antibody, anti small inducible cytokine A3-like 1, MIP-1aP antibody, anti MGC104178 antibody, anti MGC182017 antibody, anti MIP1AP antibody

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Protocol Information

ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

CCL3 and CCL3L1 Antibody (orb19059)

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100 μg
$ 320.00
DispatchUsually dispatched within 5-10 working days
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