CCKA Receptor (mouse) Antibody

SKU: orb19509

Description

Goat polyclonal antibody to Cckar

Research Area

Neuroscience

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:64000, WB: 0.1-0.3 μg/ml
• Reactivity: Rat
• Predicted Reactivity: Mouse
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Approx. 75kDa band observed in purified membranes of Rat insulinoma cell line RIN14B (calculated MW of 48.4kDa according to NP_033957.1). Data were published by El-Kouhen K and Morisset J. Int J Endocrinol. 2009;2009:875167; PMID: 20011057. The observed molecular weight also corresponds to earlier findings in literature with different antibodies (Morisset et al J Histochem Cytochem. 2003 Nov;51(11):1501-13; PMID: 14566022). Recommended concentration: 0.1-0.3 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: CCKA Receptor (mouse)
• Protein Sequence: KFDASQKKSAKEKR
• Molecular Weight: 48.4
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti Cckar antibody, anti cholecystokinin A receptor antibody, anti AW106902 antibody

Quick Database Links

Gene Symbol

CCKA Receptor (mouse)