CBX3/HP1 Gamma Antibody

SKU: orb19108

Description

Goat polyclonal antibody to CBX3

Images & Validation

• Tested Applications: ELISA, FC, IF, IHC, WB
• Dilution range: ELISA: 1:32000, WB: 0.3-1.0 μg/ml, IHC-P: 2-3μg/ml, IF/ICC: 10μg/ml, FACS: 10ug/ml
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Canine, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.IHC: In paraffin embedded Human Placenta shows strong nuclear staining in trophoblasts. Recommended concentration, 2-3ug/ml.WB: Approx. 20kDa band observed in lysates of A431, HeLa and HepG2. (calculated MW of 20.8kDa according to NP_009207.2 and NP_057671.2). Recommended concentration: 0.3-1.0 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: CBX3 / HP1 Gamma
• Molecular Weight: 20.8
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Disclaimer: For research use only

Alternative Names

anti CBX3 antibody, anti HP1-GAMMA antibody, anti chromobox homolog 3 (HP1 gamma homolog, Drosophila) antibody, anti HGNC:1553 antibody, anti HECH antibody, anti HP1Hs-gamma antibody, anti HP1 gamma homolog antibody, anti chromobox homolog 3 antibody, anti chromobox homolog 3 (Drosophila HP1 gamma) antibody, anti heterochromatin protein HP1 gamma antibody, anti heterochromatin-like protein 1 antibody

Western blot analysis of NIH3T3 nuclear cell lysate using CBX3 antibody.

Immunohistochemical staining of Human Placenta using CBX3 antibody.

Flow cytometric analysis of paraformaldehyde fixed HeLa cells using CBX3 antibody.

Immunofluorescence analysis of paraformaldehyde-fixed U2OS cells using CBX3 antibody.

Immunofluorescence analysis of paraformaldehyde fixed HeLa cells using CBX3 antibody.

Western blot analysis of A431 (A), HeLa (B), HepG2 (C), Jurkat (D) and K562 (E) nuclear cell lysate using CBX3 antibody.

Quick Database Links

Gene Symbol

CBX3 / HP1 Gamma

NCBI Gene ID

11335