CBX1/HP1-Beta Antibody

SKU: orb19107

Description

Goat polyclonal antibody to CBX1

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:32000, WB: 0.3-1.0 μg/ml, IHC-P: 2-3µg/ml
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.IHC: In paraffin embedded Human Placenta shows strong nuclear staining in trophoblasts. Recommended concentration, 2-3ug/ml.WB: Approx. 25kDa band observed in lysates of A431, HeLa and HepG2 (calculated MW of 25kDa according to NP_006798.1). Recommended concentration: 0.3-1.0 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: CBX1 / HP1-Beta
• Molecular Weight: 21.4
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Disclaimer: For research use only

Alternative Names

anti CBX1 antibody, anti HP1-BETA antibody, anti chromobox homolog 1 (HP1 beta homolog Drosophila ) antibody, anti HGNC:1551 antibody, anti CBX antibody, anti HP1Hs-beta antibody, anti M31 antibody, anti MOD1 antibody, anti chromobox homolog 1 (Drosophila HP1 beta) antibody, anti heterochromatin protein p25 beta antibody, anti HP1Hsbeta antibody, anti p25beta antibody, anti heterochromatin protein 1-beta antibody, anti modifier 1 protein antibody

orb19107 (0.3ug/ml) staining of A431 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb19107 (2.5ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Quick Database Links

Gene Symbol

CBX1 / HP1-Beta

NCBI Gene ID

10951