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CBR3 Antibody
Description
Images & Validation
−| Tested Applications | ELISA, WB |
|---|---|
| Dilution range | Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx. 35kDa band observed in Human Uterus lysates and in preliminary testing of Human Testes lysate (calculated MW of 30.9kDa according to NP_001227.1). This molecular weight has been observed by other commercial sources. Recommended concentration: 1-3µg/ml. Primary incubation 1 hour at room temperature. |
| Reactivity | Human |
| Predicted Reactivity | Bovine, Canine, Porcine |
Key Properties
−| Host | Goat |
|---|---|
| Clonality | Polyclonal |
| Target | CBR3 |
| Molecular Weight | 30.9 |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Disclaimer | For research use only |
Alternative Names
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Primary incubation 1 hour at room temperature. Image A: Human Uterus lysate at primary Ab concentration 3 ug/ml. (Loaded 35 µg protein in RIPA buffer, per lane). Detected by chemiluminescence.
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Protocol Information
CBR3 Antibody (orb18663)
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