Goat anti-CBARA1 (aa113-126) Antibody

• Catalog Number: orb125159
• Category: Antibodies
• Description: Goat polyclonal antibody to CBARA1
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Bovine, Canine, Human, Mouse, Porcine, Rat
• Dilution range: ELISA: 1:32000, WB: 0.5-2 μg/ml, IHC-P: 3.75 μg/ml
• Conjugation: Unconjugated
• MW: 54.6; 54.4
• Target: CBARA1 (aa113-126)
• Entrez: 10367
• Protein Sequence: EYENRIRAYSTPDK
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti CBARA1 antibody, anti calcium binding atopy-r
• Note: For research use only
• Application notes: WB: Approx 55kDa band observed in lysates of cell line K562 (calculated MW of 54.6kDa according to NP_006068.2). Recommended concentration: 0.5-2µg/ml. An additional band of unknown identity was also consistently observed at 37kDa. This band was successfully . IHC: Paraffin embedded Human Lung. Recommended concentration: 3.75µg/ml.
• Expiration Date: 12 months from date of receipt.

orb125159 (3.75µg/ml) staining of paraffin embedded Human Lung. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb125159 (0.5µg/ml) staining of K562 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.