CBARA1 (aa113-126) Antibody

SKU: orb125159

Description

Goat polyclonal antibody to CBARA1

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:32000, WB: 0.5-2 μg/ml, IHC-P: 3.75 μg/ml
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Porcine, Rat
• Application Notes:
  WB: Approx 55kDa band observed in lysates of cell line K562 (calculated MW of 54.6kDa according to NP_006068.2). Recommended concentration: 0.5-2µg/ml. An additional band of unknown identity was also consistently observed at 37kDa. This band was successfully . IHC: Paraffin embedded Human Lung. Recommended concentration: 3.75µg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: CBARA1 (aa113-126)
• Molecular Weight: 54.6; 54.4
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Disclaimer: For research use only

Alternative Names

anti CBARA1 antibody, anti calcium binding atopy-related autoantigen 1 antibody, anti CALC antibody, anti DKFZp564C246 antibody, anti EFHA3 antibody, anti FLJ12684 antibody, anti OTTHUMP00000019787 antibody, anti ara CALC antibody, anti atopy related autoantigen antibody, anti calcium-binding atopy-related autoantigen 1 antibody

orb125159 (3.75µg/ml) staining of paraffin embedded Human Lung. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb125159 (0.5µg/ml) staining of K562 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Quick Database Links

Gene Symbol

CBARA1 (aa113-126)

NCBI Gene ID

10367