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Cathepsin F Antibody

SKU: orb19041

Description

Goat polyclonal antibody to CTSF

Images & Validation

Tested ApplicationsELISA, WB
Dilution rangeELISA: 1:32000, WB: 0.3-1 μg/ml
ReactivityHuman
Predicted ReactivityCanine, Mouse, Rat
Application Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Experiments gave bands at approx 30kDa and 35kDa in Human Prostate and Ovary lysates after 0.3 μg/ml antibody staining. These bands correspond to earlier findings in literature with different antibodies (Wang et al, J Biol Chem. 1998 Nov 27;273(48):32000-8; PMID: 9822672). This protein has a calculated MW of 53.4kDa according to NP_003784.2. Recommended concentration: 0.3-1 μg/ml.

Key Properties

HostGoat
ClonalityPolyclonal
TargetCathepsin F
Molecular Weight53.4
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
DisclaimerFor research use only

Alternative Names

anti CTSF antibody, anti cathepsin F antibody, anti HGNC:2531 antibody, anti CATSF antibody

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Cathepsin F Antibody

Western blot analysis of Human Ovary lysate using CTSF antibody

NCBI Gene Details

No NCBI Gene data available

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Cathepsin F Antibody (orb19041)

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Available Sizes

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100 μg
$ 500.00
DispatchUsually dispatched within 5-10 working days
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