Goat anti-Cathepsin B Antibody

SKU: orb613764

Description

Goat polyclonal antibody to CTSB

Research Area

Neuroscience

Images & Validation

• Tested Applications: ELISA, FC, IF, IHC, WB
• Dilution Range: ELISA: 1:64000, WB: 0.5-2 μg/ml, IHC-P: 5 μg/ml
• Reactivity: Human
• Application Notes:
  WB: Approx 35kDa band observed in lysates of cell line HepG2 and in Human Lung, Human Colo-rectal carcinoma and Human Brain Glio-Blastoma cancer lysates (calculated MW of 37.8xkDa according to NP_001899.1). Recommended concentration: 0.5-2µg/ml. Primary incu. IHC: Paraffin embedded Human Prostate. Recommended concentration: 5µg/ml.. Notes: Immunofluorescence: Strong expression of the protein seen in the cytoplasm/ vesicles of HeLa cells. Recommended concentration: 10µg/ml. Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration:

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: Cathepsin B
• Protein Sequence: SDRICIHTNAHVSVE
• Molecular Weight: 37.8
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Anti-CTSB antibody, anti-cathepsin B antibody, anti-APPS antibody, anti-CPSB antibody, anti-APP secretase antibody, anti-amyloid precursor protein secretase antibody, anti-cathepsin B1 antibody, anti-cysteine protease antibody

Quick Database Links

Gene Symbol

Cathepsin B

NCBI Gene ID

1508