Goat anti-CARD6 Antibody

• Catalog Number: orb18496
• Category: Antibodies
• Description: Goat polyclonal antibody to CARD6
• Target: CARD6
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: ATESTPSEIIERER
• RRID: AB_10752571
• MW: 116
• Tested applications: ELISA, IHC
• Dilution range: ELISA: 1:32000, IHC-P: 5-10µg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.IHC: In paraffin embedded Human Pancreas shows variable staining of cytoplasm in acinar cells. Recommended concentration, 5-10ug/ml.WB: Approx. 45-50kDa and 25-30kDa bands observed in A549, Human Heart and Human Skeletal Muscle lysates at 1ug of primary. Please note that the bands we observe are different from the predicted size of approx. 119kDa according to NP_115976. Currently we cannot find an explanation for this in the literature. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any splice variants/modified forms been reported
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti CARD6 antibody, anti caspase recruitment doma
• Note: For research use only
• Entrez: 84674

Immunohistochemical staining of Human Pancreas using CARD6 antibody