Goat anti-Calponin 2 / CNN2 Antibody

• Catalog Number: orb20283
• Category: Antibodies
• Description: Goat polyclonal antibody to CNN2
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Human
• Dilution range: ELISA: 1:32000, WB: 0.03-0.1 μg/ml, IHC-P: 2.5 μg/ml
• Conjugation: Unconjugated
• MW: 33.7; 29.5
• Target: Calponin 2 / CNN2
• Entrez: 1265
• Protein Sequence: DPGEVPEYPPYYQEE
• RRID: AB_10750516
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti CNN2 antibody, anti calponin 2 antibody, anti
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 35kDa band observed in lysates of cell line HepG2 (calculated MW of 33.7kDa according to NP_004359.1). Recommended concentration: 0.03-0.1 μg/ml.
• Expiration Date: 12 months from date of receipt.

orb20283 (0.03ug/ml) staining of HepG2 lysates (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.