Calcipressin-1 Antibody

SKU: orb12369

Description

Goat polyclonal antibody to RCAN1

Images & Validation

• Tested Applications: ELISA, FC, IF, WB
• Dilution range: ELISA: 1:32000, WB: 1-3 μg/ml
• Reactivity: Mouse
• Predicted Reactivity: Bovine, Canine, Human, Rat
• Application Notes:
  WB: Approx 40kDa band corresponding to isoform 1L observed in Mouse Brain lysates and this band is not present in the KO mouse. Additional bands around 55kDa were consistently observed, however these band were also observed in the KO mice and therefore they

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: Calcipressin-1
• Molecular Weight: 28.1; 13.3; 22.8
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Disclaimer: For research use only

Alternative Names

anti RCAN1 antibody, anti regulator of calcineurin 1 antibody, anti ADAPT78 antibody, anti CSP1 antibody, anti DSC1 antibody, anti DSCR1 antibody, anti MCIP1 antibody, anti RCN1 antibody, anti Down syndrome candidate region 1 antibody, anti OTTHUMP00000108621 antibody, anti OTTHUMP00000108622 antibody, anti OTTHUMP00000214669 antibody, anti OTTHUMP00000214670 antibody, anti calcipressin-1 antibody, anti calcium and antibody

1 μg/ml staining of Mouse Brain and KO Mouse Brain lysates (35 μg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

1 μg/ml staining of Human Cerebellum lysate (A) + peptide (B). (35 μg protein in RIPA buffer). Detected by chemiluminescence.

Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml).

Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml).

Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (1 μg/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Quick Database Links

Gene Symbol

Calcipressin-1

NCBI Gene ID

1827