Calcipressin-1 Antibody

• Catalog Number: orb12369
• Category: Antibodies
• Description: Goat polyclonal antibody to RCAN1
• Target: Calcipressin-1
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Mouse
• Predicted Reactivity: Bovine, Canine, Human, Rat
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: HIGSSHLAPPNPD
• MW: 28.1; 13.3; 22.8
• Tested applications: ELISA, FC, IF, WB
• Dilution range: ELISA: 1:32000, WB: 1-3 μg/ml
• Application notes: WB: Approx 40kDa band corresponding to isoform 1L observed in Mouse Brain lysates and this band is not present in the KO mouse. Additional bands around 55kDa were consistently observed, however these band were also observed in the KO mice and therefore they
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti RCAN1 antibody, anti regulator of calcineurin
• Note: For research use only
• Entrez: 1827

1 μg/ml staining of Mouse Brain and KO Mouse Brain lysates (35 μg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

1 μg/ml staining of Human Cerebellum lysate (A) + peptide (B). (35 μg protein in RIPA buffer). Detected by chemiluminescence.

Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml).

Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml).

Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (1 μg/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.