calbindin D28 Antibody

• Catalog Number: orb12289
• Category: Antibodies
• Description: Goat Polyclonal antibody to Calbindin
• Target: calbindin D28
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human, Rat
• Predicted Reactivity: Bovine, Canine, Porcine
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: KTFVDQYGQRDDGK
• MW: 30
• Tested applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:128000, WB: 0.03-0.1 μg/ml, IHC-P: 5 μg/ml
• Application notes: WB: Approx. 26kDa band observed in Human and Rat Kidney lysates and in Rat Brain lysates (calculated MW of 30.0kDa according to Human NP_004920.1and Rat NP_114190.1). The observed molecular weight is routinely observed by other sources. Recommended concentrat. IHC: Paraffin embedded Human Kidney. Recommended concentration: 5-7µg/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: CALB1; calbindin 1, 28kDa; CALB; D-28K; OTTHUMP000
• Note: For research use only
• Entrez: 793

Western blot analysis of staining of Human Cerebellum lysate using CALB1 antibody.

Immunohistochemical analysis of staining of Human Cerebellum lysate using Calbindin antibody

Western blot analysis of staining of Mouse (A) and Rat (B) Brain lysates using CALB1 antibody.

Immunohistochemical staining of Human Kidney using Calbindin antibody