Goat anti-C2GnT-M (aa 410 to 422) Antibody

• Catalog Number: orb19711
• Category: Antibodies
• Description: Goat polyclonal antibody to GCNT3
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Canine, Human, Mouse, Rat
• Dilution range: ELISA: 1:64000, WB: 0.1-0.3 μg/ml
• Conjugation: Unconjugated
• MW: 50.9
• Target: C2GnT-M (aa 410 to 422)
• Entrez: 9245
• Protein Sequence: NKFDPKVDDNALQ
• RRID: AB_10748998
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti GCNT3 antibody, anti glucosaminyl (N-acetyl)
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Approx 55-60kDa band observed in Human Duodenum lysates (calculated MW of 50.9kDa according to NP_004742.1). In transfected HEK293 transiently expressing GCNT3 a band of approx. 55kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.1-0.3 μg/ml.
• Expiration Date: 12 months from date of receipt.

orb19711 (0.1ug/ml) staining of Human Duodenum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

HEK293 overexpressing GCNT3 (RC202007) and probed with orb19711 (mock transfection in first lane), tested by Origene.